Title

Type I and Type III Collagen Content of Healing Wounds in Fetal and Adult Rats.

Publication/Presentation Date

4-1-1988

Abstract

Full-thickness, dermal wounds were surgically created on the dorsa of fetal rats on the 17th day of gestation. The granulation tissue which developed after 2 days (19 days of gestation) was harvested from six to nine animals and pooled and the collagen was extracted with 0.5 M acetic acid and acetic acid plus pepsin. The ratio of type III:type I collagen was estimated from densitometer scans of electrophoretically separated alpha-chains. Full-thickness (to fascia depth) wounds were also produced on the dorsa of adult rats and granulation tissue which had developed for different periods of time up to 30 days was excised. Relative proportions of type III and type I collagen were assessed in normal and granulation tissues taken from the adult rats. Both fetal and adult granulation tissues have elevated type III collagen content but normal fetal tissue has a much higher content of type III than does normal adult tissue.

Volume

187

Issue

4

First Page

493

Last Page

497

ISSN

0037-9727

Disciplines

Other Medical Specialties | Surgery

PubMedID

3353398

Department(s)

Department of Surgery, Department of Surgery Faculty

Document Type

Article