Improved Procedure for Determination of Serum Lipid-Associated Sialic Acid: Application for Early Diagnosis of Colorectal Cancer.

Publication/Presentation Date

10-19-1988

Abstract

We have developed a simple and reliable procedure for determining serum levels of lipid-associated sialic acid (LASA) in crude preparations. This method extracts essentially all gangliosides, excludes glycoprotein-bound sialic acid, and gives LASA values (0.5-1.0 mg/dL) in good agreement with values for isolated serum gangliosides. The procedure was used to determine serum levels of LASA in patients with colorectal cancer, in patients with nonmalignant diseases (pathological control subjects), and in normal control subjects. The results indicated that the percentages of total sialic acid (TSA) comprised by LASA (LASA/TSA X 100) were elevated in patients with the earliest stages of colorectal cancer, compared with percentages in normal control subjects (P less than .001) and pathological control subjects (P less than .01).

Volume

80

Issue

16

First Page

1333

Last Page

1337

ISSN

0027-8874

Disciplines

Other Medical Specialties | Surgery

PubMedID

3172258

Department(s)

Department of Surgery, Department of Surgery Faculty

Document Type

Article

Link to Full Text

Share

COinS