Skeletal Muscle Bioenergetics in Critical Limb Ischemia and Diabetes.
Publication/Presentation Date
8-1-2023
Abstract
INTRODUCTION: Mitochondrial dysfunction is implicated in the metabolic myopathy accompanying peripheral artery disease (PAD) and critical limb ischemia (CLI). Type-2 diabetes mellitus (T2DM) is a major risk factor for PAD development and progression to CLI and may also independently be related to mitochondrial dysfunction. We set out to determine the effect of T2DM in the relationship between CLI and muscle mitochondrial respiratory capacity and coupling control.
METHODS: We studied CLI patients undergoing revascularization procedures or amputation, and non-CLI patients with or without T2DM of similar age. Mitochondrial respiratory capacity and function were determined in lower limb permeabilized myofibers by high-resolution respirometry.
RESULTS: Fourteen CLI patients (65 ± 10y) were stratified into CLI patients with (n = 8) or without (n = 6) T2DM and were compared to non-CLI patients with (n = 18; 69 ± 5y) or without (n = 19; 71 ± 6y) T2DM. Presence of CLI but not T2DM had a marked impact on all mitochondrial respiratory states in skeletal muscle, adjusted for the effects of sex. Leak respiration (State 2, P < 0.025 and State 4
CONCLUSIONS: Skeletal muscle mitochondrial respiratory capacity was blunted by ∼35% in patients with CLI. T2DM was not associated with muscle oxidative capacity and did not moderate the relationship between muscle mitochondrial respiratory capacity and CLI.
Volume
288
First Page
108
Last Page
117
ISSN
1095-8673
Published In/Presented At
Rontoyanni, V. G., Blears, E., Nunez Lopez, O., Ogunbileje, J., Moro, T., Bhattarai, N., Randolph, A. C., Fry, C. S., Fankhauser, G. T., Cheema, Z. F., Murton, A. J., Volpi, E., Rasmussen, B. B., & Porter, C. (2023). Skeletal Muscle Bioenergetics in Critical Limb Ischemia and Diabetes. The Journal of surgical research, 288, 108–117. https://doi.org/10.1016/j.jss.2023.02.015
Disciplines
Medicine and Health Sciences
PubMedID
36963297
Department(s)
Fellows and Residents
Document Type
Article