Digital Polymerase Chain Reaction Paired with High-Speed Atomic Force Microscopy for Quantitation and Length Analysis of DNA Length Polymorphisms.
DNA length polymorphisms are found in many serious diseases, and assessment of their length and abundance is often critical for accurate diagnosis. However, measuring their length and frequency in a mostly wild-type background, as occurs in many situations, remains challenging due to their variable and repetitive nature. To overcome these hurdles, we combined two powerful techniques, digital polymerase chain reaction (dPCR) and high-speed atomic force microscopy (HSAFM), to create a simple, rapid, and flexible method for quantifying both the size and proportion of DNA length polymorphisms. In our approach, individual amplicons from each dPCR partition are imaged and sized directly. We focused on internal tandem duplications (ITDs) located within the
Published In/Presented At
Koebley, S. R., Mikheikin, A., Leslie, K., Guest, D., McConnell-Wells, W., Lehman, J. H., Al Juhaishi, T., Zhang, X., Roberts, C. H., Picco, L., Toor, A., Chesney, A., & Reed, J. (2020). Digital Polymerase Chain Reaction Paired with High-Speed Atomic Force Microscopy for Quantitation and Length Analysis of DNA Length Polymorphisms. ACS nano, 14(11), 15385–15393. https://doi.org/10.1021/acsnano.0c05897
Medicine and Health Sciences
Department of Medicine, Hematology-Medical Oncology Division