DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle.
Progress in whole-genome sequencing using short-read (e.g., <150 >bp), next-generation sequencing technologies has reinvigorated interest in high-resolution physical mapping to fill technical gaps that are not well addressed by sequencing. Here, we report two technical advances in DNA nanotechnology and single-molecule genomics: (1) we describe a labeling technique (CRISPR-Cas9 nanoparticles) for high-speed AFM-based physical mapping of DNA and (2) the first successful demonstration of using DVD optics to image DNA molecules with high-speed AFM. As a proof of principle, we used this new "nanomapping" method to detect and map precisely BCL2-IGH translocations present in lymph node biopsies of follicular lymphoma patents. This HS-AFM "nanomapping" technique can be complementary to both sequencing and other physical mapping approaches.
Published In/Presented At
Mikheikin, A., Olsen, A., Leslie, K., Russell-Pavier, F., Yacoot, A., Picco, L., Payton, O., Toor, A., Chesney, A., Gimzewski, J. K., Mishra, B., & Reed, J. (2017). DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle. Nature communications, 8(1), 1665. https://doi.org/10.1038/s41467-017-01891-9
Medicine and Health Sciences
Department of Medicine, Hematology-Medical Oncology Division