Sperm Motility, Morphology, Hyperactivation, and Ionophore-Induced Acrosome Reactions After Overnight Incubation with Mycoplasmas.
OBJECTIVE: To evaluate the effect on the function on human sperm of overnight incubation with mycoplasma species.
DESIGN: Prospective controlled in vitro study.
SETTING: Community-based sperm bank and andrology laboratory.
INTERVENTIONS: Percoll-separated sperm were incubated overnight with a low densities of Myco-plasma hominis and Ureaplasma urealyticum serotypes.
MAIN OUTCOME MEASURES: Motility, midpiece and tail morphology, hyperactivation after overnight capacitation, and acrosome status after incubation with calcium ionophore.
RESULTS: Overnight incubation with mycoplasma species resulted in small but highly statistically significant differences in motility, morphology, hyperactivation after overnight capacitation, and proportion acrosome reacted after incubation with calcium ionophore compared with controls.
CONCLUSIONS: Incubation with mycoplasma species impairs sperm physiology. Identification and treatment of mycoplasma may augment a physiology-based infertility evaluation.
Published In/Presented At
Rose, B. I., & Scott, B. (1994). Sperm motility, morphology, hyperactivation, and ionophore-induced acrosome reactions after overnight incubation with mycoplasmas. Fertility And Sterility, 61(2), 341-348.
Obstetrics and Gynecology
Department of Obstetrics and Gynecology