Tissue-directed Implantation Using Ultrasound Visualization for Development of Biologically Relevant Metastatic Tumor Xenografts.
Publication/Presentation Date
1-1-2017
Abstract
BACKGROUND: Advances in cancer therapeutics depend on reliable in vivo model systems. To develop biologically relevant xenografts, ultrasound was utilized for tissue-directed implantation of neuroblastoma (NB) cell line and patient-derived tumors in the adrenal gland, and for renal subcapsular engraftment of Ewing's sarcoma (ES).
MATERIALS AND METHODS: NB xenografts were established by direct adrenal injection of luciferase-transfected NB cell lines (IMR32, SH-SY5Y, SK-N-BE2) or NB patient-derived tumor cells (UMNBL001, UMNBL002). ES xenografts were established by renal subcapsular injection of TC32, A673, CHLA-25, or A4573 cells. Progression was monitored by in vivo imaging.
RESULTS: Tumors progressed to local disease with metastasis evident by 5 weeks. Metastatic sites included cortical bone, lung, liver, and lymph nodes. Xenografted tumors retained immunochemical features of the original cancer.
CONCLUSION: Human NB adrenal xenografts, including two patient-derived orthotopic, and ES renal subcapsular xenografts were established by ultrasound without open surgery. Tissue-directed implantation is an effective technique for developing metastatic preclinical models.
Volume
31
Issue
5
First Page
779
Last Page
791
ISSN
1791-7549
Published In/Presented At
VAN Noord, R. A., Thomas, T., Krook, M., Chukkapalli, S., Hoenerhoff, M. J., Dillman, J. R., Lawlor, E. R., Opipari, V. P., & Newman, E. A. (2017). Tissue-directed Implantation Using Ultrasound Visualization for Development of Biologically Relevant Metastatic Tumor Xenografts. In vivo (Athens, Greece), 31(5), 779–791. https://doi.org/10.21873/invivo.11131
Disciplines
Medicine and Health Sciences
PubMedID
28882943
Department(s)
Department of Surgery
Document Type
Article