Neutrophil IL-10 suppresses peritoneal inflammatory monocytes during polymicrobial sepsis.
Publication/Presentation Date
3-1-2011
Abstract
Septic peritonitis remains a major cause of death. Neutrophils and inflammatory monocytes are principal components of the innate immune system and are essential for defense against a range of microbial pathogens. Their role and interaction in polymicrobial sepsis have not been defined clearly. Using a murine model of CLP to induce moderate sepsis, we found that neutrophil depletion did not alter survival, whereas depletion of neutrophils and inflammatory monocytes markedly reduced survival. After neutrophil depletion, inflammatory monocytes had greater phagocytic capacity and oxidative burst, and increased expression of costimulatory molecules, TNF, and iNOS. Notably, peritoneal neutrophils produced IL-10 following CLP. Adoptive i.p. transfer of WT but not IL-10(-/-) neutrophils into septic mice reduced monocyte expression of TNF. In vitro experiments confirmed that monocyte suppression was mediated by neutrophil-derived IL-10. Thus, during septic peritonitis, neutrophils suppress peritoneal inflammatory monocytes through IL-10 and are dispensable for survival.
Volume
89
Issue
3
First Page
423
Last Page
432
ISSN
1938-3673
Published In/Presented At
Ocuin, L. M., Bamboat, Z. M., Balachandran, V. P., Cavnar, M. J., Obaid, H., Plitas, G., & DeMatteo, R. P. (2011). Neutrophil IL-10 suppresses peritoneal inflammatory monocytes during polymicrobial sepsis. Journal of leukocyte biology, 89(3), 423–432. https://doi.org/10.1189/jlb.0810479
Disciplines
Medicine and Health Sciences
PubMedID
21106642
Department(s)
Department of Surgery, Lehigh Valley Topper Cancer Institute
Document Type
Article