A patient-derived explant (PDE) model of hormone-dependent cancer.
Publication/Presentation Date
9-1-2018
Abstract
Breast and prostate cancer research to date has largely been predicated on the use of cell lines in vitro or in vivo. These limitations have led to the development of more clinically relevant models, such as organoids or murine xenografts that utilize patient-derived material; however, issues related to low take rate, long duration of establishment, and the associated costs constrain use of these models. This study demonstrates that ex vivo culture of freshly resected breast and prostate tumor specimens obtained from surgery, termed patient-derived explants (PDEs), provides a high-throughput and cost-effective model that retains the native tissue architecture, microenvironment, cell viability, and key oncogenic drivers. The PDE model provides a unique approach for direct evaluation of drug responses on an individual patient's tumor, which is amenable to analysis using contemporary genomic technologies. The ability to rapidly evaluate drug efficacy in patient-derived material has high potential to facilitate implementation of personalized medicine approaches.
Volume
12
Issue
9
First Page
1608
Last Page
1622
ISSN
1878-0261
Published In/Presented At
Centenera, M. M., Hickey, T. E., Jindal, S., Ryan, N. K., Ravindranathan, P., Mohammed, H., Robinson, J. L., Schiewer, M. J., Ma, S., Kapur, P., Sutherland, P. D., Hoffmann, C. E., Roehrborn, C. G., Gomella, L. G., Carroll, J. S., Birrell, S. N., Knudsen, K. E., Raj, G. V., Butler, L. M., & Tilley, W. D. (2018). A patient-derived explant (PDE) model of hormone-dependent cancer. Molecular oncology, 12(9), 1608–1622. https://doi.org/10.1002/1878-0261.12354
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
30117261
Department(s)
Administration and Leadership
Document Type
Article