Transcriptional organization of the Clostridium acetobutylicum genome.
Publication/Presentation Date
1-1-2004
Abstract
Prokaryotic genes are frequently organized in multicistronic operons (or transcriptional units, TUs), and usually the regulatory motifs for the whole TU are located upstream of the first TU gene. Although the number of sequenced genomes has increased dramatically, experimental information on TU organization is extremely limited. Even for organisms as extensively studied as Escherichia coli and Bacillus subtilis, TU annotation is far from complete. It therefore becomes imperative to rely on computational approaches to complement experimental information. Here we present a TU map for the obligate anaerobe Clostridium acetobutylicum ATCC 824. This map is largely based on the distance between pairs of consecutive genes but enhanced and refined by predictions of several types of promoters (sigmaA, sigmaE and sigmaF/G) and rho-independent terminator structures. Based on the set of known C.acetobutylicum TUs, the presented TU map offers an 88% prediction accuracy.
Volume
32
Issue
6
First Page
1973
Last Page
1981
ISSN
1362-4962
Published In/Presented At
Paredes, C. J., Rigoutsos, I., & Papoutsakis, E. T. (2004). Transcriptional organization of the Clostridium acetobutylicum genome. Nucleic acids research, 32(6), 1973–1981. https://doi.org/10.1093/nar/gkh509
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
15060177
Department(s)
Administration and Leadership
Document Type
Article