RNase κ promotes robust piRNA production by generating 2',3'-cyclic phosphate-containing precursors.
Publication/Presentation Date
7-23-2021
Abstract
In animal germlines, PIWI proteins and the associated PIWI-interacting RNAs (piRNAs) protect genome integrity by silencing transposons. Here we report the extensive sequence and quantitative correlations between 2',3'-cyclic phosphate-containing RNAs (cP-RNAs), identified using cP-RNA-seq, and piRNAs in the Bombyx germ cell line and mouse testes. The cP-RNAs containing 5'-phosphate (P-cP-RNAs) identified by P-cP-RNA-seq harbor highly consistent 5'-end positions as the piRNAs and are loaded onto PIWI protein, suggesting their direct utilization as piRNA precursors. We identified Bombyx RNase Kappa (BmRNase κ) as a mitochondria-associated endoribonuclease which produces cP-RNAs during piRNA biogenesis. BmRNase κ-depletion elevated transposon levels and disrupted a piRNA-mediated sex determination in Bombyx embryos, indicating the crucial roles of BmRNase κ in piRNA biogenesis and embryonic development. Our results reveal a BmRNase κ-engaged piRNA biogenesis pathway, in which the generation of cP-RNAs promotes robust piRNA production.
Volume
12
Issue
1
First Page
4498
Last Page
4498
ISSN
2041-1723
Published In/Presented At
Shigematsu, M., Kawamura, T., Morichika, K., Izumi, N., Kiuchi, T., Honda, S., Pliatsika, V., Matsubara, R., Rigoutsos, I., Katsuma, S., Tomari, Y., & Kirino, Y. (2021). RNase κ promotes robust piRNA production by generating 2',3'-cyclic phosphate-containing precursors. Nature communications, 12(1), 4498. https://doi.org/10.1038/s41467-021-24681-w
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
34301931
Department(s)
Administration and Leadership
Document Type
Article