Guanylyl cyclase C messenger RNA is a biomarker for recurrent stage II colorectal cancer.
Publication/Presentation Date
12-7-1999
Abstract
BACKGROUND: Patients with stage II colorectal cancer and no histologic evidence of lymph node invasion develop recurrent disease, presumably because of undetected micrometastases. Guanylyl cyclase C is expressed by intestinal and colorectal cancer cells but not by extraintestinal tissues or tumors.
OBJECTIVE: To examine the expression of guanylyl cyclase C messenger RNA (mRNA) in lymph nodes of patients with node-negative colorectal cancer who did and did not have recurrent disease.
DESIGN: Case-control study.
SETTING: Tertiary care academic medical center.
PATIENTS: Paraffin-embedded lymph nodes were obtained from 21 patients with histologically confirmed node-negative colorectal cancer who had undergone resection. Controls included 11 patients without disease recurrence 6 or more years after resection, and case-patients included 10 patients whose disease recurred up to 3 years after resection.
MEASUREMENTS: Sections of paraffin-embedded lymph nodes were obtained from each patient and were pooled, and their RNA was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR).
RESULTS: Guanylyl cyclase C mRNA was expressed in lymph nodes from all patients with recurrent disease but not in those from patients without recurrent disease (P = 0.004). Nested RT-PCR that used primers for carcinoembryonic antigen, a marker for colorectal cancer, identified carcinoembryonic antigen mRNA in lymph nodes from only 1 of 10 patients with recurrent disease and those from 0 of 11 patients without recurrent disease. The odds ratio for death associated with expression of guanylyl cyclase C mRNA in regional lymph nodes was 15.0 (95% CI, 1.1 to 756.7).
CONCLUSIONS: Expression of guanylyl cyclase C mRNA in lymph nodes is associated with recurrence of colorectal cancer in patients with stage II disease. Analysis of guanylyl cyclase mRNA expression by RT-PCR may be useful for colorectal cancer staging.
Volume
131
Issue
11
First Page
805
Last Page
812
ISSN
0003-4819
Published In/Presented At
Cagir, B., Gelmann, A., Park, J., Fava, T., Tankelevitch, A., Bittner, E. W., Weaver, E. J., Palazzo, J. P., Weinberg, D., Fry, R. D., & Waldman, S. A. (1999). Guanylyl cyclase C messenger RNA is a biomarker for recurrent stage II colorectal cancer. Annals of internal medicine, 131(11), 805–812. https://doi.org/10.7326/0003-4819-131-11-199912070-00002
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
10610624
Department(s)
Administration and Leadership
Document Type
Article