Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia.
Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.
Published In/Presented At
Souders, C. A., Maynard, S. E., Yan, J., Wang, Y., Boatright, N. K., Sedan, J., & ... Simas, T. M. (2015). Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia. International Journal Of Molecular Sciences, 16(6), 12436-12453. doi:10.3390/ijms160612436
Medical Sciences | Medicine and Health Sciences
Department of Medicine, Department of Medicine Faculty