Routine Mitogenome MPS Analysis from 1 and 5 mm of Rootless Human Hair.
Publication/Presentation Date
11-18-2022
Abstract
While hair shafts are a common evidence type in forensic cases, they are often excluded from DNA analysis due to their limited DNA quantity and quality. Mitochondrial (mt) DNA sequencing is the method of choice when working with rootless hair shaft fragments due to the elevated copy number of mtDNA and the highly degraded nature of nuclear (n) DNA. Using massively parallel sequencing (MPS) of the mitochondrial (mito) genome, we studied the impact of hair age (time since collection) and physical characteristics (hair diameter, medullary structure, and length of hair tested) on mtDNA recovery and MPS data quality. Hair shaft cuttings of 1 and 5 mm from hairs less than five years to 46 years of age from 60 donors were characterized microscopically. Mitogenome sequences were generated using the Promega PowerSeq
Volume
13
Issue
11
ISSN
2073-4425
Published In/Presented At
Canale, L. C., McElhoe, J. A., Dimick, G., DeHeer, K. M., Beckert, J., & Holland, M. M. (2022). Routine Mitogenome MPS Analysis from 1 and 5 mm of Rootless Human Hair. Genes, 13(11), 2144. https://doi.org/10.3390/genes13112144
Disciplines
Medicine and Health Sciences
PubMedID
36421819
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article