Title

PCR Amplification of 16S rDNA From Lyophilized Cell Cultures Facilitates Studies in Molecular Systematics.

Publication/Presentation Date

1-1-1990

Abstract

The sequence of the major portion of a Bacillus cycloheptanicus strain SCH(T) 16S rRNA gene is reported. This sequence suggests that B. cycloheptanicus is genetically quite distinct from traditional Bacillus strains (e.g., B. subtilis) and may be properly regarded as belonging to a different genus. The sequence was determined from DNA that was produced by direct amplification of ribosomal DNA from a lyophilized cell pellet with straightforward polymerase chain reaction (PCR) procedures. By obviating the need to revive cell cultures from the lyophile pellet, this approach facilitates rapid 16S rDNA sequencing and thereby advances studies in molecular systematics.

Volume

21

First Page

325

Last Page

327

ISSN

0343-8651

Disciplines

Medical Pathology | Pathology

PubMedID

11538071

Department(s)

Department of Pathology and Laboratory Medicine, Pathology Laboratory Medicine Faculty

Document Type

Article

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