The Human Gastrin/Cholecystokinin Type B Receptor Gene: Alternative Splice Donor Site In Exon 4 Generates Two Variant mRNAs.
Gastrin and its carboxyl-terminal homolog cholecystokinin (CCK) exert a variety of biological actions in the brain and gastrointestinal tract that are mediated in part through one or more G protein-coupled receptors which exhibit similar affinity for both peptides. Genomic clones encoding a human gastrin/CCKB receptor were isolated by screening a human EMBL phage library with a partial-length DNA fragment which was based on the nucleotide sequence of the canine gastrin receptor. The gene contained a 1356-bp open reading frame consisting of five exons interrupted by 4 introns and was assigned to human chromosome 11p15.4. A region of exon 4, which encodes a portion of the putative third intracellular loop, appears to be alternatively spliced to yield two different mRNAs, one containing (452 amino acids; long isoform) and the other lacking (447 amino acids; short isoform) the pentapeptide sequence Gly-Gly-Ala-Gly-Pro. The two receptor isoforms may contribute to functional differences in gastrin- and CCK-mediated signal transduction.
Published In/Presented At
Song, I., Brown, D. R., Wiltshire, R. N., Gantz, I., Trent, J. M., and Yamada, T. (1993). The human gastrin/cholecystokinin type B receptor gene: alternative splice donor site in exon 4 generates two variant mRNAs. Proceedings Of The National Academy Of Sciences Of The United States Of America, 90(19), 9085-9089.
Medical Pathology | Pathology
Department of Pathology and Laboratory Medicine, Pathology Laboratory Medicine Faculty