Effects of Ca(II) ions on Mn(II) dynamics in chick glia and rat astrocytes: potential regulation of glutamine synthetase.
Previous studies have demonstrated that in glia and astrocytes Mn(II) is distributed with ca. 30-40% in the cytoplasm, 60-70% in mitochondria. Ca(II) ions were observed to alter both the flux rates and distribution of Mn(II) ions in primary cultures of chick glia and rat astrocytes. External (influxing) Ca(II) ions had the greatest effect on Mn(II) uptake and efflux, compared to internal (effluxing) or internal-external equilibrated Ca(II) ions. External (influxing) Ca(II) ions inhibited the net rate and extent of Mn(II) uptake but enhanced Mn(II) efflux from mitochondria. These observations differ from Ca(II)-Mn(II) effects previously reported with "brain" (neuronal) mitochondria. Overall, increased cytoplasmic Ca(II) acts to block Mn(II) uptake and enhance Mn(II) release by mitochondria, which serve to increase the cytoplasmic concentration of free Mn(II). A hypothesis is presented involving external L-glutamate acting through membrane receptors to mobilize cell Ca(II), which in turn causes mitochondrial Mn(II) to be released. Because the concentration of free cytoplasmic Mn(II) is poised near the Kd for Mn(II) with glutamine synthetase, a slight increase in cytoplasmic Mn(II) will directly enhance the activity of glutamine synthetase, which catalyzes removal of neurotoxic glutamate and ammonia.
Published In/Presented At
Wedler, F. C., Vichnin, M. C., Ley, B. W., Tholey, G., Ledig, M., & Copin, J. C. (1994). Effects of Ca(II) ions on Mn(II) dynamics in chick glia and rat astrocytes: potential regulation of glutamine synthetase. Neurochemical research, 19(2), 145–151. https://doi.org/10.1007/BF00966809
Diagnosis | Medicine and Health Sciences | Other Analytical, Diagnostic and Therapeutic Techniques and Equipment | Radiology
Department of Radiology and Diagnostic Medical Imaging