Novel immunotherapeutic effects of topically administered ripasudil (K-115) on corneal allograft survival.
Corneal allograft survival is mediated by the variety of immunological reactions and wound healing process. Our aim was to explore the effects of topical administration of ripasudil, a selective Rho-associated coiled-coil protein kinase inhibitor, on corneal allograft survival. Ripasudil was administered to mice thrice a day after allogeneic corneal transplantation. Corneal graft survival, opacity, neovascularization, re-epithelization, immune cell infiltration, and mRNA levels of angiogenic and pro-inflammatory factors in the grafted cornea and draining lymph nodes (dLNs) were evaluated with slit-lamp microscopy, immunohistochemistry, flow cytometry, and polymerase chain reaction. Graft survival was significantly prolonged with lower graft opacity and neovascularization scores in 0.4% and 2.0% ripasudil-treated groups, and mRNA levels of angiogenic and pro-inflammatory factors in ripasudil-treated grafted corneas were reduced. Moreover, 0.4% and 2.0% ripasudil reduced CD45
Published In/Presented At
Inomata, T., Fujimoto, K., Okumura, Y., Zhu, J., Fujio, K., Shokirova, H., Miura, M., Okano, M., Funaki, T., Sung, J., Negishi, N., & Murakami, A. (2020). Novel immunotherapeutic effects of topically administered ripasudil (K-115) on corneal allograft survival. Scientific reports, 10(1), 19817. https://doi.org/10.1038/s41598-020-76882-w
Medical Education | Medicine and Health Sciences
USF-LVHN SELECT Program, USF-LVHN SELECT Program Students