Biological effects of stored platelet-rich plasma eye-drops in corneal wound healing.
BACKGROUND/AIMS: This study aimed to assess the efficacy and sterility of stored platelet-rich plasma (PRP) eye-drops for corneal epithelial wound healing compared with those of autologous serum (AS) eye-drops.
METHODS: At our single institution, PRP and AS eye-drops were prepared using peripheral blood obtained from six healthy volunteers and stored at 4°C. Platelet and leucocyte counts and transforming growth factor (TGF)-β1, epidermal growth factor (EGF), and fibronectin levels were assessed during storage for up to 4 weeks. Sterility was assessed by culturing 4-week poststorage samples. PRP, AS, and phosphate-buffered saline (PBS) eye-drop efficacies were compared using corneal epithelial wound healing assays
RESULTS: Higher platelet and lower leucocyte counts were seen in PRP than in whole blood on the day of preparation. After storage, TGF-β1, EGF, and fibronectin levels were significantly higher in PRP than in AS eye-drops.
CONCLUSION: PRP eye-drops stored at 4°C for 4 weeks promoted corneal epithelial wound healing with higher levels of growth factors than those observed in AS eye-drops, while maintaining sterility, suggesting that this preparation satisfies the unmet medical needs in the treatment of refractory keratoconjunctival epithelial disorders.
Published In/Presented At
Okumura, Y., Inomata, T., Fujimoto, K., Fujio, K., Zhu, J., Yanagawa, A., Shokirova, H., Saita, Y., Kobayashi, Y., Nagao, M., Nishio, H., Sung, J., Midorikawa-Inomata, A., Eguchi, A., Nagino, K., Akasaki, Y., Hirosawa, K., Huang, T., Kuwahara, M., & Murakami, A. (2022). Biological effects of stored platelet-rich plasma eye-drops in corneal wound healing. The British journal of ophthalmology, bjo-2022-322068. Advance online publication. https://doi.org/10.1136/bjo-2022-322068
Medical Education | Medicine and Health Sciences
USF-LVHN SELECT Program, USF-LVHN SELECT Program Students