USF-LVHN SELECT
A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing.
Publication/Presentation Date
3-27-2019
Abstract
G protein-coupled receptors (GPCRs) are central to how mammalian cells sense and respond to chemicals. Mammalian olfactory receptors (ORs), the largest family of GPCRs, mediate the sense of smell through activation by small molecules, though for most bonafide ligands, they have not been identified. Here, we introduce a platform to screen large chemical panels against multiplexed GPCR libraries using next-generation sequencing of barcoded genetic reporters in stably engineered human cell lines. We mapped 39 mammalian ORs against 181 odorants and identified 79 interactions that have not been reported to our knowledge, including ligands for 15 previously orphaned receptors. This multiplexed receptor assay allows the cost-effective mapping of large chemical libraries to receptor repertoires at scale.
Volume
8
Issue
3
First Page
254
Last Page
260
ISSN
2405-4720
Published In/Presented At
Jones, E. M., Jajoo, R., Cancilla, D., Lubock, N. B., Wang, J., Satyadi, M., Cheung, R., de March, C., Bloom, J. S., Matsunami, H., & Kosuri, S. (2019). A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing. Cell systems, 8(3), 254–260.e6. https://doi.org/10.1016/j.cels.2019.02.009
Disciplines
Medical Education | Medicine and Health Sciences
PubMedID
30904378
Department(s)
USF-LVHN SELECT Program, USF-LVHN SELECT Program Students
Document Type
Article