The effect of a fibroblast derived skin substitute on keratinocyte proliferation

Publication/Presentation Date

2006

Abstract

Introduction: Patients with partial thickness thermal injuries are often treated with a fibroblast derived skin substitute to provide immediate wound closure, improve pain control, and accelerate healing. Previous clinical studies have demonstrated that a fibroblast derived temporary skin substitute (TransCyte®) can accelerate wound healing in partial thickness burn wounds. The purpose of this study was to examine the effects of a fibroblast derived temporary skin substitute on keratinocyte proliferation in a split thickness skin graft model. Methods: Twelve female athymic mice were divided into two groups. Group 1 had 3:1 meshed split thickness skin grafts placed on their backs and covered with Biobrane®. Group 2 had 3:1 meshed split thickness grafts placed on their backs and covered with TransCyte. Tissue samples were taken on post op days 4, 8, 13. The monoclonal antibody Ki-67 was then used to stain for mitotic keratinocytes in the proliferating graft edge. Results: The number of actively dividing keratinocytes in the migrating tongue of epithelium at the graft edge were counted at post op days 4, 8, 13. On POD4 group 1 had mean of 13.6 cells and group 2 had a mean of 19.4 cells. On POD8 group 1 had mean of 29.3 cells and group 2 had mean of 43.6 cells. On POD13 group 1 had a mean of 15.7 cells and group 2 had a mean of 45.4 cells. There was a significant difference between both groups on all post op days (P =

Volume

33

Issue

1

First Page

s62

Last Page

s63

Comments

Abstract Presented at: The 13th International Society for Burn Injuries Congress, Brazil. 2006 September 24-29.

Department(s)

Department of Surgery, Department of Surgery Faculty

Document Type

Article

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