Title

The effect of a fibroblast derived skin substitute on keratinocyte proliferation

Publication/Presentation Date

2006

Abstract

Introduction: Patients with partial thickness thermal injuries are often treated with a fibroblast derived skin substitute to provide immediate wound closure, improve pain control, and accelerate healing. Previous clinical studies have demonstrated that a fibroblast derived temporary skin substitute (TransCyte®) can accelerate wound healing in partial thickness burn wounds. The purpose of this study was to examine the effects of a fibroblast derived temporary skin substitute on keratinocyte proliferation in a split thickness skin graft model. Methods: Twelve female athymic mice were divided into two groups. Group 1 had 3:1 meshed split thickness skin grafts placed on their backs and covered with Biobrane®. Group 2 had 3:1 meshed split thickness grafts placed on their backs and covered with TransCyte. Tissue samples were taken on post op days 4, 8, 13. The monoclonal antibody Ki-67 was then used to stain for mitotic keratinocytes in the proliferating graft edge. Results: The number of actively dividing keratinocytes in the migrating tongue of epithelium at the graft edge were counted at post op days 4, 8, 13. On POD4 group 1 had mean of 13.6 cells and group 2 had a mean of 19.4 cells. On POD8 group 1 had mean of 29.3 cells and group 2 had mean of 43.6 cells. On POD13 group 1 had a mean of 15.7 cells and group 2 had a mean of 45.4 cells. There was a significant difference between both groups on all post op days (P = <0.001). Conclusion: These findings suggest that the placement of TransCyte on meshed grafts can accelerate keratinocyte proliferation as indicated by increased number of mitotically active keratinocytes in the migrating epithelium. This may explain how TransCyte functions to accelerate wound healing in partial thickness burns. There was a significant increase in proliferation when compared to Biobrane which demonstrates the advantage of the dermal matrix deposited by the fibroblasts

Volume

33

Issue

1

First Page

s62

Last Page

s63

Comments

Abstract Presented at: The 13th International Society for Burn Injuries Congress, Brazil. 2006 September 24-29.

Department(s)

Department of Surgery, Department of Surgery Faculty

Document Type

Article

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