Title

Sperm Motility, Morphology, Hyperactivation, and Ionophore-Induced Acrosome Reactions After Overnight Incubation with Mycoplasmas.

Publication/Presentation Date

2-1-1994

Abstract

OBJECTIVE: To evaluate the effect on the function on human sperm of overnight incubation with mycoplasma species.

DESIGN: Prospective controlled in vitro study.

SETTING: Community-based sperm bank and andrology laboratory.

INTERVENTIONS: Percoll-separated sperm were incubated overnight with a low densities of Myco-plasma hominis and Ureaplasma urealyticum serotypes.

MAIN OUTCOME MEASURES: Motility, midpiece and tail morphology, hyperactivation after overnight capacitation, and acrosome status after incubation with calcium ionophore.

RESULTS: Overnight incubation with mycoplasma species resulted in small but highly statistically significant differences in motility, morphology, hyperactivation after overnight capacitation, and proportion acrosome reacted after incubation with calcium ionophore compared with controls.

CONCLUSIONS: Incubation with mycoplasma species impairs sperm physiology. Identification and treatment of mycoplasma may augment a physiology-based infertility evaluation.

Volume

61

Issue

2

First Page

341

Last Page

348

ISSN

0015-0282

Disciplines

Obstetrics and Gynecology

PubMedID

8299794

Department(s)

Department of Obstetrics and Gynecology

Document Type

Article