Sperm Motility, Morphology, Hyperactivation, and Ionophore-Induced Acrosome Reactions After Overnight Incubation with Mycoplasmas.
Publication/Presentation Date
2-1-1994
Abstract
OBJECTIVE: To evaluate the effect on the function on human sperm of overnight incubation with mycoplasma species.
DESIGN: Prospective controlled in vitro study.
SETTING: Community-based sperm bank and andrology laboratory.
INTERVENTIONS: Percoll-separated sperm were incubated overnight with a low densities of Myco-plasma hominis and Ureaplasma urealyticum serotypes.
MAIN OUTCOME MEASURES: Motility, midpiece and tail morphology, hyperactivation after overnight capacitation, and acrosome status after incubation with calcium ionophore.
RESULTS: Overnight incubation with mycoplasma species resulted in small but highly statistically significant differences in motility, morphology, hyperactivation after overnight capacitation, and proportion acrosome reacted after incubation with calcium ionophore compared with controls.
CONCLUSIONS: Incubation with mycoplasma species impairs sperm physiology. Identification and treatment of mycoplasma may augment a physiology-based infertility evaluation.
Volume
61
Issue
2
First Page
341
Last Page
348
ISSN
0015-0282
Published In/Presented At
Rose, B. I., & Scott, B. (1994). Sperm motility, morphology, hyperactivation, and ionophore-induced acrosome reactions after overnight incubation with mycoplasmas. Fertility And Sterility, 61(2), 341-348.
Disciplines
Obstetrics and Gynecology
PubMedID
8299794
Department(s)
Department of Obstetrics and Gynecology
Document Type
Article