Modeling shear damage to suspended CHO cells during cross-flow filtration.
Publication/Presentation Date
1-1-2007
Abstract
A mathematical model is presented for predicting the shear-induced decrease in live cells occurring over time during tangential flow filtration. The model uses a cell death rate constant (K) and considers the effects of flow rate, solution viability, and filtration system volumes and dimensions. Single pass and recycle capillary experiments with solutions of high (93%), medium (87%), and low (70%) viability were run, where the maximum laminar shear stress ranged from 10- 300 Pa, to validate the model and determine cell death rate constants. The K values for the suspended CHO cells used in this research ranged from 0.06 to 12.5 s-1. These K values increased with shear stress, as expected, and also as the solution viability decreased.
Volume
23
Issue
1
First Page
194
Last Page
199
ISSN
1520-6033
Published In/Presented At
Vickroy, B., Lorenz, K., & Kelly, W. (2007). Modeling shear damage to suspended CHO cells during cross-flow filtration. Biotechnology progress, 23(1), 194–199. https://doi.org/10.1021/bp060183e
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
17269688
Department(s)
Administration and Leadership
Document Type
Article