A chronic model to simultaneously measure intracranial pressure, cerebral blood flow, and study the pial microvasculature.
Publication/Presentation Date
8-22-1997
Abstract
In an effort to study changes in cerebral blood flow (CBF), intracranial pressure (ICP) and intracranial compliance (ICC) simultaneously, we have developed a chronic model in rats using a pial window crown with two ports. This model can also be used to study vasoreactivity of pial vessels. Female Sprague-Dawley rats weighing between 225-250 g underwent placement of cranial chamber with dual ports under pentobarbital anesthesia. To test the utility of this technique 45 groups of rats were studied. Group 1 consisted of control animals. Group 2 consisted of rats undergoing 15 min of global cerebral ischemia. Rats in group 3 were evaluated for changes in vessel diameter and ICP after adenosine injection. In group 4 leukocyte/endothelial interactions were evaluated. These groups demonstrate the ability of this model to monitor CBF, ICP, ICC and pial vessel architecture in chronic rat experiments.
Volume
75
Issue
2
First Page
155
Last Page
160
ISSN
0165-0270
Published In/Presented At
Jallo, J., Saetzler, R., Mishke, C., Young, W. F., Vasthare, U., & Tuma, R. F. (1997). A chronic model to simultaneously measure intracranial pressure, cerebral blood flow, and study the pial microvasculature. Journal of neuroscience methods, 75(2), 155–160. https://doi.org/10.1016/s0165-0270(97)00064-2
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
9288647
Department(s)
Administration and Leadership
Document Type
Article