Identification of a novel Bcl-2 related gene, BRAG-1, in human glioma.
Publication/Presentation Date
3-7-1996
Abstract
Programmed cell death (apoptosis) plays a major role in embryogenesis, in mature organ homeostasis and in many disease states including cancer. Apoptosis occurs as an orderly cell-intrinsic suicide program regulated by a family of genes related to Bcl-2. Here, we describe the cloning and molecular characterization of a gene homologous to Bcl-2 from a human glioma. This gene named BRAG-1 (for brain-related apoptosis gene) has an open reading frame that encodes for a protein of 31 kDa sharing significant sequence homology with the Bcl-2 family of genes in the BH1 and BH2 regions. Northern blot analyses revealed that BRAG-1 is expressed in human gliomas as a 1.8 kb message. This gene, interestingly, was found to be expressed predominantly in normal human brain as a 4.5 kb transcript which is different in size from the message found in tumor tissues. These results suggest that BRAG-1 may be rearranged in human gliomas leading to its over-expression as a truncated transcript. Utilizing a bacterial expression vector, we produced BRAG-1 protein which was found to cross-react with a Bcl-2 monoclonal antibody, further suggesting structural and immunological similarity to Bcl-2.
Volume
12
Issue
5
First Page
947
Last Page
951
ISSN
0950-9232
Published In/Presented At
Das, R., Reddy, E. P., Chatterjee, D., & Andrews, D. W. (1996). Identification of a novel Bcl-2 related gene, BRAG-1, in human glioma. Oncogene, 12(5), 947–951.
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
8649811
Department(s)
Administration and Leadership
Document Type
Article