Manual Hippocampal Subfield Segmentation Using High-Field MRI: Impact of Different Subfields in Hippocampal Volume Loss of Temporal Lobe Epilepsy Patients.

Authors

• Jose Eduardo Peixoto-Santos
• Luciana Estefani Drumond de Carvalho
• Ludmyla Kandratavicius
• Paula Rejane Beserra Diniz
• Renata Caldo Scandiuzzi
• Roland Coras
• Ingmar Blümcke
• Joao Alberto Assirati
• Carlos Gilberto Carlotti
• Caio Cesar Marconato Simoes Matias
• Carlos Ernesto Garrido Salmon
• Antonio Carlos Dos Santos
• Tonicarlo R Velasco
• Marcio Flavio D Moraes
• Joao Pereira Leite

Publication/Presentation Date

1-1-2018

Abstract

In patients with temporal lobe epilepsy (TLE), presurgical magnetic resonance imaging (MRI) often reveals hippocampal atrophy, while neuropathological assessment indicates the different types of hippocampal sclerosis (HS). Different HS types are not discriminated in MRI so far. We aimed to define the volume of each hippocampal subfield on MRI manually and to compare automatic and manual segmentations for the discrimination of HS types. The T2-weighted images from 14 formalin-fixed age-matched control hippocampi were obtained with 4.7T MRI to evaluate the volume of each subfield at the anatomical level of the hippocampal head, body, and tail. Formalin-fixed coronal sections at the level of the body of 14 control cases, as well as tissue samples from 24 TLE patients, were imaged with a similar high-resolution sequence at 3T. Presurgical three-dimensional (3D) T1-weighted images from TLE went through a FreeSurfer 6.0 hippocampal subfield automatic assessment. The manual delineation with the 4.7T MRI was identified using Luxol Fast Blue stained 10-μm-thin microscopy slides, collected at every millimeter. An additional section at the level of the body from controls and TLE cases was submitted to NeuN immunohistochemistry for neuronal density estimation. All TLE cases were classified according to the International League Against Epilepsy's (ILAE's) HS classification. Manual volumetry in controls revealed that the dentate gyrus (DG)+CA4 region, CA1, and subiculum accounted for almost 90% of the hippocampal volume. The manual 3T volumetry showed that all TLE patients with type 1 HS (TLE-HS1) had lower volumes for DG+CA4, CA2, and CA1, whereas those TLE patients with HS type 2 (TLE-HS2) had lower volumes only in CA1 (

Volume

9

First Page

927

Last Page

927

ISSN

1664-2295

Published In/Presented At

Peixoto-Santos, J. E., de Carvalho, L. E. D., Kandratavicius, L., Diniz, P. R. B., Scandiuzzi, R. C., Coras, R., Blümcke, I., Assirati, J. A., Carlotti, C. G., Matias, C. C. M. S., Salmon, C. E. G., Dos Santos, A. C., Velasco, T. R., Moraes, M. F. D., & Leite, J. P. (2018). Manual Hippocampal Subfield Segmentation Using High-Field MRI: Impact of Different Subfields in Hippocampal Volume Loss of Temporal Lobe Epilepsy Patients. Frontiers in neurology, 9, 927. https://doi.org/10.3389/fneur.2018.00927

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

30524352

Department(s)

Administration and Leadership

Document Type

Article