Expression of MicroRNAs miR-145, miR-181c, miR-199a and miR-1183 in the Blood and Hippocampus of Patients with Mesial Temporal Lobe Epilepsy.

Authors

• Luana Grupioni Lourenço Antônio
• Priscila Freitas-Lima
• Gabriela Pereira-da-Silva
• João Alberto Assirati
• Caio Marconato Matias
• Mucio Luiz Assis Cirino
• Luis Fernando Tirapelli
• Tonicarlo Rodrigues Velasco
• Americo Ceiki Sakamoto
• Carlos Gilberto Carlotti
• Daniela Pretti da Cunha Tirapelli

Publication/Presentation Date

12-1-2019

Abstract

The aim of this study was to analyze the expression profiles of the microRNAs (miRNAs) miR-145, miR-181c, miR-199a and miR-1183 in the hippocampus and blood of patients with mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS) and to investigate whether these can be used as diagnosis and prognosis biomarkers for epilepsy. Hippocampus and blood samples were collected from 20 patients with MTLE-HS, ten of whom had a favorable surgical outcome (Engel I) and ten with an unfavorable surgical outcome (Engel III-IV). Hippocampus samples from autopsied individuals with no neurological or psychiatric medical history (necropsy samples) and blood samples from healthy individuals were used as controls. Real-time quantitative PCR (RQ-PCR) was used to analyze miRNA expression. The results showed that the expressions of these miRNAs differed quantitatively in the hippocampus and blood of patients with MTLE-HS in comparison to the respective control. This difference was most pronounced for miR-145, which was hypo-expressed in the hippocampus and hyper-expressed in the blood of MTLE-HS patients. MiRNAs miR-145, miR-181c, miR-199a and miR-1183 were hyper-expressed in the blood of patients with MTLE-HS. No statistical differences in the levels of these miRNAs in the blood or hippocampus were found between Engel I patients and Engel III-IV patients. These results suggest that the analyzed microRNAs are potential circulating biomarkers for epilepsy diagnosis.

Volume

69

Issue

4

First Page

580

Last Page

587

ISSN

1559-1166

Published In/Presented At

Antônio, L. G. L., Freitas-Lima, P., Pereira-da-Silva, G., Assirati, J. A., Jr, Matias, C. M., Cirino, M. L. A., Tirapelli, L. F., Velasco, T. R., Sakamoto, A. C., Carlotti, C. G., Jr, & Tirapelli, D. P. D. C. (2019). Expression of MicroRNAs miR-145, miR-181c, miR-199a and miR-1183 in the Blood and Hippocampus of Patients with Mesial Temporal Lobe Epilepsy. Journal of molecular neuroscience : MN, 69(4), 580–587. https://doi.org/10.1007/s12031-019-01386-w

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

31368064

Department(s)

Administration and Leadership

Document Type

Article