Protein transfer of preformed MHC-peptide complexes sensitizes target cells to T cell cytolysis.
Publication/Presentation Date
10-1-1994
Abstract
Recombinant GPI-anchored HLA-A2.1 (HLA-A2.1-GPI/beta 2m) was used as a protein transfer vehicle to deliver a hepatitis B virus antigenic peptide to the surfaces of cytotoxic T cell targets. Empty HLA-A2.1-GPI/beta 2m was first produced in D. melanogaster cotransfectants and immunoaffinity purified. Cell coating with HLA-A2.1-GPI/beta 2m was shown to occur rapidly, and to be protein concentration dependent. Protein-transferred HLA-A2.1-GPI/beta 2m effectively presented a hepatitis B virus peptide to peptide-specific HLA-A2.1-restricted T cell clones in cytotoxicity assays. Protein transfer of functional GPI-modified class I MHC-antigenic peptide complexes represents a novel strategy for delivering functional antigenic complexes to cell surfaces that bypasses limitations of gene transfer and permits control of antigenic peptide densities at cell surfaces.
Volume
1
Issue
7
First Page
607
Last Page
613
ISSN
1074-7613
Published In/Presented At
Huang, J. H., Getty, R. R., Chisari, F. V., Fowler, P., Greenspan, N. S., & Tykocinski, M. L. (1994). Protein transfer of preformed MHC-peptide complexes sensitizes target cells to T cell cytolysis. Immunity, 1(7), 607–613. https://doi.org/10.1016/1074-7613(94)90050-7
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
7600289
Department(s)
Administration and Leadership
Document Type
Article