Directional antisense and sense cDNA cloning using Epstein-Barr virus episomal expression vectors.
Publication/Presentation Date
9-30-1989
Abstract
A set of Epstein-Barr virus (EBV) episomal expression vectors, incorporating either the Rous sarcoma virus 3' long terminal repeat or the human metallothionein IIA gene promoter, were constructed. The transcriptional cassettes encompassed by these vectors were designed to permit both antisense and sense RNA transcription. A novel methodology was developed for directional cDNA cloning using an oligodeoxyribonucleotide adapter; the EBV episomal vectors alternatively enabled the insertion of cDNA segments in antisense or sense orientations. We propose a strategy for random antisense RNA mutagenesis exploiting this vector system and a method for episome-based directional antisense cDNA cloning and expression, permitting the rapid identification of genes mediating selectable cellular functions.
Volume
81
Issue
2
First Page
285
Last Page
294
ISSN
0378-1119
Published In/Presented At
Groger, R. K., Morrow, D. M., & Tykocinski, M. L. (1989). Directional antisense and sense cDNA cloning using Epstein-Barr virus episomal expression vectors. Gene, 81(2), 285–294. https://doi.org/10.1016/0378-1119(89)90189-3
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
2478421
Department(s)
Administration and Leadership
Document Type
Article