Role of amyloid precursor protein (APP): study with antisense transfection of human neuroblastoma cells.
Publication/Presentation Date
4-1-1992
Abstract
The function of amyloid precursor protein (APP) was investigated in human neuroblastoma La-N-1 cells by stable transfection with a DNA construct encoding antisense APP mRNA. Levels of APP mRNA, as well as proteins, were reduced by 80-90% in antisense APP transfected (ASAT) cells. ASAT cells exhibited three main features as a result of APP gene expression deprivation: (1) a 30% reduction in cell proliferation, (2) reduced cell adhesion that could be reversed by the addition of La-N-1 conditioned media as a source of secreted APP, and (3) a two- and four-fold increase in neurite-bearing cells suggesting that cellular APP may be involved in neurite extension. The first two features confirm previously reported functions for APP in proliferation and adhesion of non-neuronal cell types but the use of neuroblastoma cells in this study disclose a novel role for cellular APP in neurite extension.
Volume
31
Issue
4
First Page
635
Last Page
645
ISSN
0360-4012
Published In/Presented At
LeBlanc, A. C., Kovacs, D. M., Chen, H. Y., Villaré, F., Tykocinski, M., Autilio-Gambetti, L., & Gambetti, P. (1992). Role of amyloid precursor protein (APP): study with antisense transfection of human neuroblastoma cells. Journal of neuroscience research, 31(4), 635–645. https://doi.org/10.1002/jnr.490310407
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
1578514
Department(s)
Administration and Leadership
Document Type
Article