Use of an Epstein-Barr virus episomal replicon for anti-sense RNA-mediated gene inhibition in a human cytotoxic T-cell clone.
Publication/Presentation Date
6-1-1988
Abstract
A methodology was developed for stable gene transfer into cloned nontransformed human T lymphocytes. Stable high-level gene expression was achieved in cloned human T cells by using a self-replicating Epstein-Barr virus (EBV) episomal replicon. A comparison of five eukaryotic promoters established that the Rous sarcoma virus 3' long terminal repeat (RSV 3' LTR) and the lymphopapilloma virus (LPV) 5' LTR are optimal for episome-based expression in T cells. Effective (greater than 95%), selective, and reversible anti-sense RNA-mediated gene inhibition of a model T-cell-associated molecule (CD8) was achieved in a cytotoxic human T-cell clone by using an EBV episome-based, RSV 3' LTR-driven expression system. The linking of anti-sense RNA mutagenesis and T-cell cloning technologies should contribute significantly to studies of human T-cell function.
Volume
85
Issue
11
First Page
4010
Last Page
4014
ISSN
0027-8424
Published In/Presented At
Hambor, J. E., Hauer, C. A., Shu, H. K., Groger, R. K., Kaplan, D. R., & Tykocinski, M. L. (1988). Use of an Epstein-Barr virus episomal replicon for anti-sense RNA-mediated gene inhibition in a human cytotoxic T-cell clone. Proceedings of the National Academy of Sciences of the United States of America, 85(11), 4010–4014. https://doi.org/10.1073/pnas.85.11.4010
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
2453884
Department(s)
Administration and Leadership
Document Type
Article