A novel class of cell surface glycolipids of mammalian cells. Free glycosyl phosphatidylinositols.
Publication/Presentation Date
5-31-1996
Abstract
Glycosyl phosphatidylinositol (GPI) lipids function as anchors of membrane proteins, and free GPI units serve as intermediates along the path of GPI-anchor biosynthesis. By using in vivo cell surface biotinylation, we show that free GPIs: 1) can exit the rough endoplasmic reticulum and are present on the surface of a murine EL-4 T-lymphoma and a human carcinoma cell (HeLa), 2) arrive at the cell surface in a time and temperature-dependent fashion, and 3) are built on a base-labile glycerol backbone, unlike GPI anchors of surface proteins of the same cells. The free GPIs described in this study may serve as a source of hormone-sensitive phosphoinositol glycans. The absence of free GPIs from the cell surface may also account for the growth advantage of blood cells in paroxysmal nocturnal hemoglobinuria.
Volume
271
Issue
22
First Page
12879
Last Page
12884
ISSN
0021-9258
Published In/Presented At
Singh, N., Liang, L. N., Tykocinski, M. L., & Tartakoff, A. M. (1996). A novel class of cell surface glycolipids of mammalian cells. Free glycosyl phosphatidylinositols. The Journal of biological chemistry, 271(22), 12879–12884. https://doi.org/10.1074/jbc.271.22.12879
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
8663031
Department(s)
Administration and Leadership
Document Type
Article