Structure of a functional rabbit class I MHC gene: similarity to human class I genes.
Publication/Presentation Date
1-1-1985
Abstract
Studies of rabbit major histocompatibility complex proteins have suggested that rabbits express only a single class I antigen, in contrast to most mouse strains (H-2K, D, and L) and man (HLA-A, -B, and -C), which express three. To explore the significance and the molecular basis of this apparent species difference, we have characterized the expressed class I protein and a corresponding cDNA clone from the rabbit cell line RL-5, which is derived from the inbred B/J rabbit strain. As an extension of these analyses, this report documents the genomic sequence of a gene, designated 19-1, which encodes the same histocompatibility antigen expressed in RL-5. The availability of the corresponding full-length cDNA and amino-terminal protein sequence indicates the fully functional nature of the 19-1 gene and allows presumptive assignment of the transcription start site and delineation of exon/intron boundaries. Comparisons of the rabbit gene with homologous human and mouse sequences reveal a striking similarity between 19-1 and human genes in both exon/intron organization and specific nucleotide sequences; this close similarity allows tentative identification of previously unrecognized transcriptional start sites in the human genes.
Volume
21
Issue
1
First Page
71
Last Page
82
ISSN
0093-7711
Published In/Presented At
Marche, P. N., Tykocinski, M. L., Max, E. E., & Kindt, T. J. (1985). Structure of a functional rabbit class I MHC gene: similarity to human class I genes. Immunogenetics, 21(1), 71–82. https://doi.org/10.1007/BF00372243
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
3917974
Department(s)
Administration and Leadership
Document Type
Article