A method for chronic membrane plasmapheresis in the rat.
Publication/Presentation Date
12-1-1993
Abstract
Therapeutic apheresis as applied to humans may encompass a single treatment or numerous treatments for disorders ranging from acute poisoning to severe chronic autoimmune disease. However, the mechanisms of beneficial effects of apheresis are not well characterized. Utilizing a miniaturized hollow-fiber membrane system, we have developed a reliable technique for long-term vascular access in the rat that permits repetitive plasmapheresis. We established vascular access in 14 animals, with 8 and 6 rats randomized to 3- and 7-wk experimental periods, respectively. Immunoglobulin levels of blood samples obtained immediately before and after each plasmapheresis were measured to examine membrane filtration characteristics. Overall, 100% of the animals survived and 93% successfully completed their assigned experimental periods. Mean decrease of immunoglobulin G and M levels for 28 plasmapheresis treatments in five rats was 66.9 +/- 8.1 and 61.0 +/- 7.3% (SD), respectively, indicating effective membrane filtration. This model of apheresis can be applied to several disorders in the rat, including, but not limited to, spontaneous insulin-dependent diabetes mellitus and experimental inflammatory bowel disease.
Volume
75
Issue
6
First Page
2820
Last Page
2824
ISSN
8750-7587
Published In/Presented At
Follette, D. B., Kerr, D., Nasca, T. J., Euler, H. H., & Pinevich, A. J. (1993). A method for chronic membrane plasmapheresis in the rat. Journal of applied physiology (Bethesda, Md. : 1985), 75(6), 2820–2824. https://doi.org/10.1152/jappl.1993.75.6.2820
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
8125906
Department(s)
Administration and Leadership
Document Type
Article