Molecular cloning, expression, and chromosomal localization of the gene encoding a human myeloid membrane antigen (gp150).
Publication/Presentation Date
10-1-1986
Abstract
DNA from a tertiary mouse cell transformant containing amplified human sequences encoding a human myeloid membrane glycoprotein, gp150, was used to construct a bacteriophage lambda library. A single recombinant phage containing 12 kilobases (kb) of human DNA was isolated, and molecular subclones were then used to isolate the complete gp150 gene from a human placental genomic DNA library. The intact gp150 gene, assembled from three recombinant phages, proved to be biologically active when transfected into NIH 3T3 cells. Molecular probes from the gp150 locus annealed with a 4.0-kb polyadenylated RNA transcript derived from human myeloid cell lines and from tertiary mouse cell transformants. The gp150 gene was assigned to human chromosome 15, and was subchromosomally localized to bands q25-26 by in situ hybridization. The chromosomal location of the gp150 gene coincides cytogenetically with the region assigned to the c-fes proto-oncogene, another human gene specifically expressed by myeloid cells.
Volume
78
Issue
4
First Page
914
Last Page
921
ISSN
0021-9738
Published In/Presented At
Look, A. T., Peiper, S. C., Rebentisch, M. B., Ashmun, R. A., Roussel, M. F., Lemons, R. S., Le Beau, M. M., Rubin, C. M., & Sherr, C. J. (1986). Molecular cloning, expression, and chromosomal localization of the gene encoding a human myeloid membrane antigen (gp150). The Journal of clinical investigation, 78(4), 914–921. https://doi.org/10.1172/JCI112680
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
2428842
Department(s)
Administration and Leadership
Document Type
Article