Murine monoclonal antibodies (MCS-1 and MCS-2) reactive with human myeloid leukemia cells.
Publication/Presentation Date
1-1-1995
Abstract
In an attempt to identify the antigens expressed on human myeloid leukemia cells, two murine monoclonal antibodies (mAb) designated as MCS-1 (isotype; IgG3) and MCS-2 (IgG1) were raised. MCS-1 reacted with peripheral blood granulocytes, but not with monocytes, whereas MCS-2 reacted with both granulocytes and monocytes. The incidence of MCS-1 and MCS-2 reactivity with the cells from a total of 121 patients with various type of leukemias was as follows: 25/46 (54%) and 39/47 (83%) in acute myeloblastic leukemia and acute monoblastic leukemia, 8/16 and 16/16 in chronic myeloid leukemia in blastic crisis (CML-BC) of myeloid type, 0/7 and 2/7 in CML-BC of lymphoid type, 0/26 and 2/32 in acute lymphoblastic leukemia (ALL), 7/7 and 7/7 in chronic phase of CML, respectively. Two cases of MCS-2 positive ALL had a Philadelphia chromosome marker (Ph1). In contrast, neither MCS-1 nor MCS-2 reacted with lymphocytes or any of leukemic and non-leukemic lymphoid cell lines tested. These results indicate that MCS-1 and MCS-2 mAb recognized two different differentiation antigens expressed on granulocytes and monocytes. These mAb would be useful reagents to determine differentiation antigens on the cells in granulocyte and monocyte lineage.
Volume
42
Issue
3
First Page
177
Last Page
185
ISSN
0023-5679
Published In/Presented At
Sagawa, K., Gouhara, R., Sudo, T., Tatsumi, E., Peiper, S. C., & Minowada, J. (1995). Murine monoclonal antibodies (MCS-1 and MCS-2) reactive with human myeloid leukemia cells. The Kurume medical journal, 42(3), 177–185. https://doi.org/10.2739/kurumemedj.42.177
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
7474856
Department(s)
Administration and Leadership
Document Type
Article