Characterization of constitutively active mutants of G protein-coupled receptors.
Publication/Presentation Date
1-1-2006
Abstract
The ability of G protein-coupled receptors to transduce signaling typically is induced by the binding of an appropriate ligand (agonist), resulting in a conformational change of the receptor and the subsequent interaction with the G protein heterotrimer. Some mutants of G protein-coupled receptors, known as constitutively active mutants, have the capacity to activate the G protein-signaling cascade even in the absence of ligand. In this chapter, we describe three methods that most directly allow characterization of constitutively active mutants and discriminate them from the wild-type receptors. All methods are based on the spontaneous signaling function in the absence of ligand and its consequences on the receptor.
Volume
332
First Page
129
Last Page
139
ISSN
1064-3745
Published In/Presented At
Navenot, J. M., Wang, Z. X., & Peiper, S. C. (2006). Characterization of constitutively active mutants of G protein-coupled receptors. Methods in molecular biology (Clifton, N.J.), 332, 129–139. https://doi.org/10.1385/1-59745-048-0:129
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
16878689
Department(s)
Administration and Leadership
Document Type
Article