Heat shock response in CHO mammalian cells is controlled by a nonlinear stochastic process.
Publication/Presentation Date
10-1-2007
Abstract
In many biological systems, the interactions that describe the coupling between different units in a genetic network are nonlinear and stochastic. We study the interplay between stochasticity and nonlinearity using the responses of Chinese hamster ovary (CHO) mammalian cells to different temperature shocks. The experimental data show that the mean value response of a cell population can be described by a mathematical expression (empirical law) which is valid for a large range of heat shock conditions. A nonlinear stochastic theoretical model was developed that explains the empirical law for the mean response. Moreover, the theoretical model predicts a specific biological probability distribution of responses for a cell population. The prediction was experimentally confirmed by measurements at the single-cell level. The computational approach can be used to study other nonlinear stochastic biological phenomena.
Volume
3
Issue
10
First Page
1859
Last Page
1870
ISSN
1553-7358
Published In/Presented At
Lipan, O., Navenot, J. M., Wang, Z., Huang, L., & Peiper, S. C. (2007). Heat shock response in CHO mammalian cells is controlled by a nonlinear stochastic process. PLoS computational biology, 3(10), 1859–1870. https://doi.org/10.1371/journal.pcbi.0030187
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
17922567
Department(s)
Administration and Leadership
Document Type
Article