Prorelaxant E-type Prostanoid Receptors Functionally Partition to Different Procontractile Receptors in Airway Smooth Muscle.

Authors

• Ajay P Nayak
• Elham Javed
• Dominic R Villalba
• Yinna Wang
• Henry P Morelli
• Sushrut D Shah
• Nicholas Kim
• Rennolds S Ostrom
• Reynold A Panettieri
• Steven S An
• Dale D Tang
• Raymond B Penn

Publication/Presentation Date

11-1-2023

Abstract

Prostaglandin E2 imparts diverse physiological effects on multiple airway cells through its actions on four distinct E-type prostanoid (EP) receptor subtypes (EP1-EP4). Gs-coupled EP2 and EP4 receptors are expressed on airway smooth muscle (ASM), yet their capacity to regulate the ASM contractile state remains subject to debate. We used EP2 and EP4 subtype-specific agonists (ONO-259 and ONO-329, respectively) in cell- and tissue-based models of human ASM contraction-magnetic twisting cytometry (MTC), and precision-cut lung slices (PCLSs), respectively-to study the EP2 and EP4 regulation of ASM contraction and signaling under conditions of histamine or methacholine (MCh) stimulation. ONO-329 was superior (< 0.05) to ONO-259 in relaxing MCh-contracted PCLSs (log half maximal effective concentration [logEC

Volume

69

Issue

5

First Page

584

Last Page

591

ISSN

1535-4989

Published In/Presented At

Nayak, A. P., Javed, E., Villalba, D. R., Wang, Y., Morelli, H. P., Shah, S. D., Kim, N., Ostrom, R. S., Panettieri, R. A., Jr, An, S. S., Tang, D. D., & Penn, R. B. (2023). Prorelaxant E-type Prostanoid Receptors Functionally Partition to Different Procontractile Receptors in Airway Smooth Muscle. American journal of respiratory cell and molecular biology, 69(5), 584–591. https://doi.org/10.1165/rcmb.2022-0445OC

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

37523713

Department(s)

Administration and Leadership

Document Type

Article