Molecular mechanism of bitter taste receptor agonist-mediated relaxation of airway smooth muscle.
Publication/Presentation Date
7-31-2024
Abstract
G-protein-coupled receptors (GPCRs) belonging to the type 2 taste receptors (TAS2Rs) family are predominantly present in taste cells to allow the perception of bitter-tasting compounds. TAS2Rs have also been shown to be expressed in human airway smooth muscle (ASM), and TAS2R agonists relax ASM cells and bronchodilate airways despite elevating intracellular calcium. This calcium "paradox" (calcium mediates contraction by pro-contractile Gq-coupled GPCRs) and the mechanisms by which TAS2R agonists relax ASM remain poorly understood. To gain insight into pro-relaxant mechanisms effected by TAS2Rs, we employed an unbiased phosphoproteomic approach involving dual-mass spectrometry to determine differences in the phosphorylation of contractile-related proteins in ASM following the stimulation of cells with TAS2R agonists, histamine (an agonist of the Gq-coupled H1 histamine receptor) or isoproterenol (an agonist of the Gs-coupled β
Volume
38
Issue
14
First Page
23842
Last Page
23842
ISSN
1530-6860
Published In/Presented At
Conaway, S., Jr, Huang, W., Hernandez-Lara, M. A., Kane, M. A., Penn, R. B., & Deshpande, D. A. (2024). Molecular mechanism of bitter taste receptor agonist-mediated relaxation of airway smooth muscle. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 38(14), e23842. https://doi.org/10.1096/fj.202400452R
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
39037554
Department(s)
Administration and Leadership
Document Type
Article