Stem cell antigen-1 expression in the pulmonary vascular endothelium.
Publication/Presentation Date
6-1-2003
Abstract
Although the function of the cell surface protein stem cell antigen-1 (Sca-1) has not been identified, expression of this molecule is a characteristic of bone marrow-derived hematopoietic stem cell populations. Expression of Sca-1, however, is not restricted to hematopoietic tissue. By RT-PCR and Western analysis, we found that Sca-1 is expressed in the adult mouse lung. Sca-1 immunohistochemistry revealed a linear staining pattern on the endothelial surface of large and small pulmonary arteries and veins and alveolar capillaries. Expression of Sca-1 in the pulmonary endothelium was confirmed by dual fluorescent microscopy on lung sections and by fluorescence-activated cell sorting analysis of digested lung tissue; each of these methods showed colocalization with the endothelial marker platelet/endothelial cell adhesion molecule-1. In the kidney, Sca-1 expression was also noted in large vessels, but, in contrast to the lung, was not observed in capillaries. Overall, our data indicate that Sca-1 expression helps define the surface phenotype of endothelial cells throughout the pulmonary vasculature.
Volume
284
Issue
6
First Page
990
Last Page
996
ISSN
1040-0605
Published In/Presented At
Kotton, D. N., Summer, R. S., Sun, X., Ma, B. Y., & Fine, A. (2003). Stem cell antigen-1 expression in the pulmonary vascular endothelium. American journal of physiology. Lung cellular and molecular physiology, 284(6), L990–L996. https://doi.org/10.1152/ajplung.00415.2002
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
12611818
Department(s)
Administration and Leadership
Document Type
Article