Adiponectin promotes macrophage polarization toward an anti-inflammatory phenotype.

Authors

• Koji Ohashi
• Jennifer L Parker
• Noriyuki Ouchi
• Akiko Higuchi
• Joseph A Vita
• Noyan Gokce
• Anette Amstrup Pedersen
• Christoph Kalthoff
• Søren Tullin
• Anette Sams
• Ross Summer
• Kenneth Walsh

Publication/Presentation Date

2-26-2010

Abstract

It is established that the adipocyte-derived cytokine adiponectin protects against cardiovascular and metabolic diseases, but the effect of this adipokine on macrophage polarization, an important mediator of disease progression, has never been assessed. We hypothesized that adiponectin modulates macrophage polarization from that resembling a classically activated M1 phenotype to that resembling alternatively-activated M2 cells. Peritoneal macrophages and the stromal vascular fraction (SVF) cells of adipose tissue isolated from adiponectin knock-out mice displayed increased M1 markers, including tumor necrosis factor-alpha, interleukin-6, and monocyte chemoattractant protein-1 and decreased M2 markers, including arginase-1, macrophage galactose N-acetyl-galactosamine specific lectin-1, and interleukin-10. The systemic delivery of adenovirus expressing adiponectin significantly augmented arginase-1 expression in peritoneal macrophages and SVF cells in both wild-type and adiponectin knock-out mice. In culture, the treatment of macrophages with recombinant adiponectin protein led to an increase in the levels of M2 markers and a reduction of reactive oxygen species and reactive oxygen species-related gene expression. Adiponectin also stimulated the expression of M2 markers and attenuated the expression of M1 markers in human monocyte-derived macrophages and SVF cells isolated from human adipose tissue. These data show that adiponectin functions as a regulator of macrophage polarization, and they indicate that conditions of high adiponectin expression may deter metabolic and cardiovascular disease progression by favoring an anti-inflammatory phenotype in macrophages.

Volume

285

Issue

9

First Page

6153

Last Page

6160

ISSN

1083-351X

Published In/Presented At

Ohashi, K., Parker, J. L., Ouchi, N., Higuchi, A., Vita, J. A., Gokce, N., Pedersen, A. A., Kalthoff, C., Tullin, S., Sams, A., Summer, R., & Walsh, K. (2010). Adiponectin promotes macrophage polarization toward an anti-inflammatory phenotype. The Journal of biological chemistry, 285(9), 6153–6160. https://doi.org/10.1074/jbc.M109.088708

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

20028977

Department(s)

Administration and Leadership

Document Type

Article