Comparing Performance of Spectral Image Analysis Approaches for Detection of Cellular Signals in Time-Lapse Hyperspectral Imaging Fluorescence Excitation-Scanning Microscopy.

Authors

• Marina Parker
• Naga S Annamdevula
• Donald Pleshinger
• Zara Ijaz
• Josephine Jalkh
• Raymond Penn
• Deepak Deshpande
• Thomas C Rich
• Silas J Leavesley

Publication/Presentation Date

5-25-2023

Abstract

Hyperspectral imaging (HSI) technology has been applied in a range of fields for target detection and mixture analysis. While HSI was originally developed for remote sensing applications, modern uses include agriculture, historical document authentication, and medicine. HSI has also shown great utility in fluorescence microscopy. However, traditional fluorescence microscopy HSI systems have suffered from limited signal strength due to the need to filter or disperse the emitted light across many spectral bands. We have previously demonstrated that sampling the fluorescence excitation spectrum may provide an alternative approach with improved signal strength. Here, we report on the use of excitation-scanning HSI for dynamic cell signaling studies-in this case, the study of the second messenger Ca

Volume

10

Issue

6

ISSN

2306-5354

Published In/Presented At

Parker, M., Annamdevula, N. S., Pleshinger, D., Ijaz, Z., Jalkh, J., Penn, R., Deshpande, D., Rich, T. C., & Leavesley, S. J. (2023). Comparing Performance of Spectral Image Analysis Approaches for Detection of Cellular Signals in Time-Lapse Hyperspectral Imaging Fluorescence Excitation-Scanning Microscopy. Bioengineering (Basel, Switzerland), 10(6), 642. https://doi.org/10.3390/bioengineering10060642

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

37370573

Department(s)

Administration and Leadership

Document Type

Article