Voltage-dependent inwardly rectifying potassium conductance in the outer membrane of neuronal mitochondria.
Publication/Presentation Date
8-27-2010
Abstract
Potassium fluxes integrate mitochondria into cellular activities, controlling their volume homeostasis and structural integrity in many pathophysiological mechanisms. The outer mitochondrial membrane (OMM) is thought to play a passive role in this process because K(+) is believed to equilibrate freely between the cytosol and mitochondrial intermembrane space. By patch clamping mitochondria isolated from the central nervous systems of adult mitoCFP transgenic mice, we discovered the existence of I(OMMKi), a novel voltage-dependent inwardly rectifying K(+) conductance located in the OMM. I(OMMKi) is regulated by osmolarity, potentiated by cAMP, and activated at physiological negative potentials, allowing K(+) to enter the mitochondrial intermembrane space in a controlled regulated fashion. The identification of I(OMMKi) in the OMM supports the notion that a membrane potential could exist across this membrane in vivo and suggests that the OMM possesses regulated pathways for K(+) uptake.
Volume
285
Issue
35
First Page
27411
Last Page
27417
ISSN
1083-351X
Published In/Presented At
Fieni, F., Parkar, A., Misgeld, T., Kerschensteiner, M., Lichtman, J. W., Pasinelli, P., & Trotti, D. (2010). Voltage-dependent inwardly rectifying potassium conductance in the outer membrane of neuronal mitochondria. The Journal of biological chemistry, 285(35), 27411–27417. https://doi.org/10.1074/jbc.M110.131243
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
20551319
Department(s)
Administration and Leadership
Document Type
Article