Chronic + binge alcohol exposure promotes inflammation and alters airway mechanics in the lung.
Publication/Presentation Date
11-1-2019
Abstract
INTRODUCTION: Alcohol use disorders are major risk factors for the development of and susceptibility to acute respiratory distress syndrome. Although these risks of alcohol consumption on the lung are well described, mechanisms by which alcohol abuse promotes acute lung injury are poorly understood. These gaps in our understanding are due, at least in part, to limitations of animal models to recapitulate human alcohol consumption. Recently, a new model of chronic plus binge alcohol exposure was developed that is hypothesized to better model drinking patterns of individuals with alcohol use disorders. Specifically, this paradigm models chronic consumption coupled with periodic bouts of heavy drinking. The impacts of this alcohol-exposure regimen on the lung are uncharacterized. Therefore, the goal of this study was to examine lung injury and inflammation in a well-characterized experimental model of chronic + binge alcohol exposure.
METHODS: 10-week-old male C57Bl6/J mice were administered ethanol-containing (or isocaloric control) liquid diet for 10 days, followed by a single ethanol gavage (5 g/kg). Lung inflammation and pulmonary function were assessed.
RESULTS: Ten days of ethanol-containing liquid diet alone (chronic) did not detectably affect any variables measured. However, ethanol diet plus gavage (chronic + binge) caused neutrophils to accumulate in the lung tissue and in the bronchoalveolar lavage fluid 24 h post-binge. This inflammatory cell recruitment was associated with airway hyper-responsiveness to inhaled methacholine, as indicated by elevated resistance, Newtonian resistance, and respiratory resistance.
CONCLUSIONS: Taken together, the novel findings reveal that ethanol alone, absent of any secondary inflammatory insult, is sufficient to produce inflammation in the lung. Although these changes were relatively mild, they were associated with functional changes in the central airways. This animal model may be useful in the future for identifying mechanisms by which alcohol abuse sensitizes at-risk individuals to lung injury.
Volume
80
First Page
53
Last Page
63
ISSN
1873-6823
Published In/Presented At
Poole, L. G., Beier, J. I., Torres-Gonzales, E., Schlueter, C. F., Hudson, S. V., Artis, A., Warner, N. L., Nguyen-Ho, C. T., Dolin, C. E., Ritzenthaler, J. D., Hoyle, G. W., Roman, J., & Arteel, G. E. (2019). Chronic + binge alcohol exposure promotes inflammation and alters airway mechanics in the lung. Alcohol (Fayetteville, N.Y.), 80, 53–63. https://doi.org/10.1016/j.alcohol.2018.10.008
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
30445135
Department(s)
Administration and Leadership
Document Type
Article