Elevated Transglutaminase-2 in SOX10-Deficient Melanoma Promotes Tumor Onset and Decreases Intratumoral CD4+ T Cells.
Publication/Presentation Date
10-1-2025
Abstract
UNLABELLED: Melanoma heterogeneity contributes to therapy resistance and immune evasion. The loss of SOX10, a neural crest lineage-specific transcription factor, leads to phenotypic switching from a proliferative cell state to an invasive, drug-tolerant cell state. SOX10-deficient cells are able to persist during immunotherapy treatment, highlighting the need to characterize the factors that regulate immune evasion downstream of SOX10 loss. In this study, we found that SOX10-deficient melanoma cell lines and patient samples express elevated levels of TGM2, a transglutaminase family member. TGM2 upregulation in SOX10 knockout cells was reversed by inhibition of epigenetic reader BET proteins. Knockdown of TGM2 did not affect the SOX10-deficient invasive cell state; however, overexpression of TGM2 in syngeneic melanomas promoted tumor onset in immunocompetent mice, but not in immunodeficient mice, suggesting an immune-mediated effect. TGM2 overexpression in melanoma was associated with decreased intratumoral CD4+ T cells, and depletion of CD4+ T cells abolished the tumor-promoting effect of TGM2. These data indicate that TGM2 is negatively regulated by SOX10 in melanoma and can promote an immunosuppressive tumor microenvironment.
SIGNIFICANCE: The transglutaminase TGM2 is negatively associated with the neural crest lineage-specific transcription factor SOX10 and is an immunomodulatory protein in cutaneous melanoma.
Volume
85
Issue
19
First Page
3614
Last Page
3632
ISSN
1538-7445
Published In/Presented At
Caksa, S., Purwin, T. J., Erkes, D. A., DeRosa, K. M., Kitterman, E., Barnada, S. M., Stefanski, C. D., Wilson, H. P., Mersky, G. L., Glasheen, M. Q., Heilizer, J. S., Ajmal, N., Sun, Y., Chan, J. S. Y., Rui, H., Chervoneva, I., Capparelli, C., & Aplin, A. E. (2025). Elevated Transglutaminase-2 in SOX10-Deficient Melanoma Promotes Tumor Onset and Decreases Intratumoral CD4+ T Cells. Cancer research, 85(19), 3614–3632. https://doi.org/10.1158/0008-5472.CAN-24-3267
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
40742313
Department(s)
Administration and Leadership
Document Type
Article