Quantification of tumor fluorescence during intraoperative optical cancer imaging.

Authors

• Ryan P Judy
• Jane J Keating
• Elizabeth M DeJesus
• Jack X Jiang
• Olugbenga T Okusanya
• Shuming Nie
• David E Holt
• Sean P Arlauckas
• Phillip S Low
• E James Delikatny
• Sunil Singhal

Publication/Presentation Date

11-13-2015

Abstract

Intraoperative optical cancer imaging is an emerging technology in which surgeons employ fluorophores to visualize tumors, identify tumor-positive margins and lymph nodes containing metastases. This study compares instrumentation to measure tumor fluorescence. Three imaging systems (Spectropen, Glomax, Flocam) measured and quantified fluorescent signal-to-background ratios (SBR) in vitro, murine xenografts, tissue phantoms and clinically. Evaluation criteria included the detection of small changes in fluorescence, sensitivity of signal detection at increasing depths and practicality of use. In vitro, spectroscopy was superior in detecting incremental differences in fluorescence than luminescence and digital imaging (Ln[SBR] = 6.8 ± 0.6, 2.4 ± 0.3, 2.6 ± 0.1, p = 0.0001). In fluorescent tumor cells, digital imaging measured higher SBRs than luminescence (6.1 ± 0.2 vs. 4.3 ± 0.4, p = 0.001). Spectroscopy was more sensitive than luminometry and digital imaging in identifying murine tumor fluorescence (SBR = 41.7 ± 11.5, 5.1 ± 1.8, 4.1 ± 0.9, p = 0.0001), and more sensitive than digital imaging at detecting fluorescence at increasing depths (SBR = 7.0 ± 3.4 vs. 2.4 ± 0.5, p = 0.03). Lastly, digital imaging was the most practical and least time-consuming. All methods detected incremental differences in fluorescence. Spectroscopy was the most sensitive for small changes in fluorescence. Digital imaging was the most practical considering its wide field of view, background noise filtering capability, and sensitivity to increasing depth.

Volume

5

First Page

16208

Last Page

16208

ISSN

2045-2322

Published In/Presented At

Judy, R. P., Keating, J. J., DeJesus, E. M., Jiang, J. X., Okusanya, O. T., Nie, S., Holt, D. E., Arlauckas, S. P., Low, P. S., Delikatny, E. J., & Singhal, S. (2015). Quantification of tumor fluorescence during intraoperative optical cancer imaging. Scientific reports, 5, 16208. https://doi.org/10.1038/srep16208

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

26563091

Department(s)

Administration and Leadership

Document Type

Article