Desmoglein 2 modulates extracellular vesicle release from squamous cell carcinoma keratinocytes.
Publication/Presentation Date
8-1-2017
Abstract
Extracellular vesicles (EVs) are nanoscale membrane-derived vesicles that serve as intercellular messengers carrying lipids, proteins, and genetic material. Substantial evidence has shown that cancer-derived EVs, secreted by tumor cells into the blood and other bodily fluids, play a critical role in modulating the tumor microenvironment and affecting the pathogenesis of cancer. Here we demonstrate for the first time that squamous cell carcinoma (SCC) EVs were enriched with the C-terminal fragment of desmoglein 2 (Dsg2), a desmosomal cadherin often overexpressed in malignancies. Overexpression of Dsg2 increased EV release and mitogenic content including epidermal growth factor receptor and c-Src. Inhibiting ectodomain shedding of Dsg2 with the matrix metalloproteinase inhibitor GM6001 resulted in accumulation of full-length Dsg2 in EVs and reduced EV release. When cocultured with Dsg2/green fluorescence protein-expressing SCC cells, green fluorescence protein signal was detected by fluorescence-activated cell sorting analysis in the CD90
Volume
31
Issue
8
First Page
3412
Last Page
3424
ISSN
1530-6860
Published In/Presented At
Overmiller, A. M., Pierluissi, J. A., Wermuth, P. J., Sauma, S., Martinez-Outschoorn, U., Tuluc, M., Luginbuhl, A., Curry, J., Harshyne, L. A., Wahl, J. K., 3rd, South, A. P., & Mahoney, M. G. (2017). Desmoglein 2 modulates extracellular vesicle release from squamous cell carcinoma keratinocytes. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(8), 3412–3424. https://doi.org/10.1096/fj.201601138RR
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
28438789
Department(s)
Administration and Leadership
Document Type
Article