Expanded RNA-binding activities of mammalian Argonaute 2.

Authors

• Grace S Tan
• Barry G Garchow
• Xuhang Liu
• Jennifer Yeung
• John P Morris
• Trinna L Cuellar
• Michael T McManus
• Marianthi Kiriakidou

Publication/Presentation Date

12-1-2009

Abstract

Mammalian Argonaute 2 (Ago2) protein associates with microRNAs (miRNAs) or small interfering RNAs (siRNAs) forming RNA-induced silencing complexes (RISCs/miRNPs). In the present work, we characterize the RNA-binding and nucleolytic activity of recombinant mouse Ago2. Our studies show that recombinant mouse Ago2 binds efficiently to miRNAs forming active RISC. Surprisingly, we find that recombinant mouse Ago2 forms active RISC using pre-miRNAs or long unstructured single stranded RNAs as guides. Furthermore, we demonstrate that, in vivo, endogenous human Ago2 binds directly to pre-miRNAs independently of Dicer, and that Ago2:pre-miRNA complexes are found both in the cytoplasm and in the nucleus of human cells.

Volume

37

Issue

22

First Page

7533

Last Page

7545

ISSN

1362-4962

Published In/Presented At

Tan, G. S., Garchow, B. G., Liu, X., Yeung, J., Morris, J. P., 4th, Cuellar, T. L., McManus, M. T., & Kiriakidou, M. (2009). Expanded RNA-binding activities of mammalian Argonaute 2. Nucleic acids research, 37(22), 7533–7545. https://doi.org/10.1093/nar/gkp812

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

19808937

Department(s)

Administration and Leadership

Document Type

Article